We study how DNA repair pathways are regulated to maintain genomic integrity
cell cycle regulation
Efficient repair of DNA damage is essential for cellular survival. When cells face DNA damage from internal or external factors, multiple steps must be taken to ensure effective repair of damage as well as restoration of wild type growth.
Our lab is broadly interested in studying the regulation of DNA damage response and repair pathways in vivo.
At present, we are addressing the following questions in microbial systems:
a. Regulation of DNA damage response and repair clearance in bacteria
How do cells recover after DNA damage onslaught
b. Regulation of pathway choice during repair
Regulation and specificity of error-prone DNA polymerases
c. Molecular mechanism of double-strand break repair via homologous recombination
How do double-strand break ends find their homologous partner
d. Mitochondrial DNA repair mechanisms
How do cells regulate mitochondrial DNA damage response and repair
We use a combination of live-cell imaging, super-resolution microscopy, high-throughput sequencing techniques as well as genetic and molecular biology assays to mechanistically understand how cells from all domains of life maintain genome integrity.
Asha Mary Joseph
Chimthanawala, A., Parmar, J. J., Kumar, S., Iyer, K. S., Rao, M., & Badrinarayanan, A. (2022). SMC protein RecN drives RecA filament translocation for in vivo homology search. Proceedings of the National Academy of Sciences, 119(46), e2209304119.
Joseph, A. M., Nahar, K., Daw, S., Hasan, M. M., Lo, R., Le, T., ... & Badrinarayanan, A. (2022). Mechanistic insight into the repair of C8-linked pyrrolobenzodiazepine monomer-mediated DNA damage. RSC Medicinal Chemistry.
Dua, Nitish, Akshaya Seshadri, and Anjana Badrinarayanan. "DarT-mediated mtDNA damage induces dynamic reorganization and selective segregation of mitochondria." Journal of Cell Biology 221.10 (2022): e202205104.
Badrinarayanan, A*, Leake, M.C. (2022). Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria. In: Leake, M.C. (eds) Chromosome Architecture. Methods in Molecular Biology, vol 2476. 978-1-0716-2221-6_4.
- Adam SB Jalal, Ngat T Tran, Clare EM Stevenson, Afroze Chimthanawala, Anjana Badrinarayanan, David M Lawson, Tung BK Le*. (2021). A CTP-dependent gating mechanism enables ParB spreading on DNA. eLife. 10:e69676.
- Joseph A, Daw S, Sadhir I and Badrinarayanan A*. (2021). Coordination between nucleotide excision repair and specialized polymerase DnaE2 action enables survival in non-replicating bacteria. eLife. 10:e67552.
Raghunathan S, Chimthanawala A, Vecchiarelli A, Krishna S and Badrinarayanan A*. (2020). Asymmetric chromosome segregation and cell division in DNA damage-induced bacterial filaments. Mol Biol Cell. mbc-E20-08-0547
Chimthanawala A., Badrinarayanan A*. (2019) "Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus Under DNA Damage". In: Badrinarayanan A. (eds) SMC Complexes. Methods in Molecular Biology, vol 2004.
Raghunathan S., Badrinarayanan A*. (2019) "Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging". In: Badrinarayanan A. (eds) SMC Complexes. Methods in Molecular Biology, vol 2004.
Badrinarayanan A, Le T, Spille J, Cisse I and Laub M. "Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB." PLoS genetics 13.5 2017. e1006783.
Badrinarayanan A and Leake M. Using Fluorescence Recovery After Photobleaching (FRAP) to study dynamics of the Structural Maintenance of Chromosome (SMC) complex in vivo. Methods in Molecular Biology, 2016. 1431:37-46.
Nolivos S, Upton A, Badrinarayanan A, Muller J, Zawadzka K, Wiktor J, GillA, Arciszewska L, Nicolas E, Sherratt D. MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation. Nature communications, 2016. 7:10466.
Badrinarayanan A, Le T, Laub M. Rapid pairing and subsequent resegregation of distant homologous loci enables DNA double-strand break repair in bacteria. The Journal of Cell Biology, 2015. 210:385-400.
Nicolas E, Uphoff S, Upton A, Henry O, Badrinarayanan A, Sherratt D. The SMC complex MukBEF recruits Topoisomerase IV to the origin of replication region in live Escherichia coli. MBio, 2014, 5(1), e1001-13.
Thank you for your interest in joining our lab! The questions we ask are inter-disciplinary in nature and we are happy to have researchers from diverse backgrounds of science working together. Do get in touch if you are interested in questions pertaining to the maintenance of genome integrity in cells.
If you are writing to me (anjana[at]ncbs[dot]res[dot]in), include a copy of your CV and a brief description of research interests (related to the work done in our lab).
At present, we do not have openings for short-term internships or MSc. thesis projects.
NATIONAL CENTRE for BIOLOGICAL SCIENCES
GKVK Campus, Bellary Road,
Bengaluru, Karnataka 560065
Phone - 080-23666001